User:Mark Skaggs/Notebook/Bacterial DNA Results

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Objective:

1. To identify unknown DNA sequence and to understand the processes of extracting said DNA.

Materials & Methods:

1. Receive code for DNA from PCR reaction in earlier lab for transect bacteria. 2. Identify bacteria species from code by using NCBI Blast website. [www.ncbi.nlm.nih.gov]

Results:

Our DNA sample did not get an accurate code and were given a code from our instructor. After using NCBI Blast, we determined that it was chryseobacteria. From classes in a previous semester using the same transect,Colette McDonnell identified two bacteria DNA strands: chryseobacteria and streptomyces. [[1]] While one of these bacteria was identical in both readings, streptomyces are unique to Transect 2.

Citations:

McConnell, Colette. "Collette McConnell's Lab Notebook." Biology 210 at American University: Spring 2014. accessed at <http://openwetware.org/wiki/User:Colette_M_McConnell/Notebook/Biology_210_at_AU>.