User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/15

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It's a beautiful life :D

  • Today, I grew 5 colonies of the J23100 + RBS + LRE + TT and 5 colonies of red click beetle luciferase to check whether the ligations were performed correctly. I incubated them for 6 hrs at 37°C.

  • After this, I extracted plasmid with the Qiagen kit for micropreps and stored them as JLRE 1-5 PP and RED 1-5 PP.

  • I also did a restriction for 20μL with ECO RI and PST I to check my ligations and I left them incubating at 37°C ON.