User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/06

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The package is here!!!


  • Today, I received the package that Cambridge team sent to us. I quickly got to work on it and continue to work in my mutated luciferase by transforming everything as follows:


1 LuxCDABE under pBad promoter (269.5 ng/µl)

2 enhanced Photinus pyralis luciferase (EPIC) and P. pyralis LRE with no promoter (142.1 ng/µl)

3 enhanced Photinus pyralis luciferase (EPIC) and P. pyralis LRE under pBad promoter(187.8 ng/µl)

4 Luciola cruciata luciferase and L. cruciata LRE with no promoter (203.1 ng/µl)

5 Luciola cruciata luciferase and L. cruciata LRE under pBad promoter (176.4 ng/µl)

6 Mut-luz in pSB1C3

7 Mut-luz with J23102

8 LRE with J23100 (Positive control)

  • I plated everything but the mut-luz with J23102 (ampicilin) and the LRE in chloramphenicol plates. And I left them incubating at 37°C ON.