User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/25

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By the way...


  • Today, I left the ligation for the mutated red firefly luciferases to insert into the dephosphated J61002 vector containing the J23102 constitutive promoter.


-H2O --------------------> 7μL

-Buffer ligase ----------> 4μL

-Vector (plasmid) -----> 2μL

-Insert (luciferase) ---> 6μL

-Ligase -----------------> 1μL