User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/09/23

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Say whaaaaat?!?!


  • Today, after inactivating the enzyme, I ran the following gel to check the restrictions:


Rest mas lucs 23Sep10.JPG

  • The lanes were as follows:


1, 15. Ladder

2, 3. Green luciferase with mutated RBS restricted with XBAI and PSTI

4, 5. Mutated Red luciferase with mutated RBS restricted with XBAI and PSTI

6,7,8. Wildtype luciferase restricted with XBAI and PSTI