User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/08/19

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We can do it!


  • As yesterday I was feeling sick, Melvin made the restriction for the LRE, which is 1127 long (or the second band observed in the gel).


Cuantificacion 19Ago10.JPG

  • The lanes were as follows:


1. Ladder

2. pSB1C3

3. J23100

4. LRE 3 restricted with XBA I and PST I

5. LRE 7 restricted with XBA I and PST I

6. LRE 3 restricted with ECO RI and PST I

7. LRE 7 restricted with ECO RI and PST I

8. TrpL

9. LuxAB

10. OmpC linear plasmid

11. OmpC restricted with ECO RI and XBA I

12. OmpF PCR

13. Co +