User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/07/01

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More hard work...


  • Today, I ran a gel to check that my restrictions and plasmids were O.K. My results weren't quite straight forward, so I will repeat the extraction of plasmids 1.2, 1.7 and 1.9 followed by PCR amplification of the plasmids.



RestrYPlasm.JPG

  • The lanes were as follows:


1. Ladder.
2. 1.2 p.
3. 1.4 p.
4. 1.6 p.
5. 1.7 p.
6. 1.9 p.
7. 2.2 p.
8. 2.2 r.
9. 2.8 p.
10. 2.8 r.
11. 3.1 p.
12. 3.1 r.
13. 3.3 p.
14. 3.3 r.
15. 3.5 p.
16. 3.5 r.
17. 3.8 p.
18. 3.8 r.
19. 3.9 p.
20. 3.9 r.
21. Ladder.
22. 4.2 p.
23. 4.2 r.
24. 4.3 p.
25. 4.3 r.
26. 4.7 p.
27. 4.7 r.
28. 4.8 p.
29. 4.8 r.
30. 4.9 p.
31. 4.9 r.


  • Cultures were incubated ON.