User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/28

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Plating


  • Today, I made new LB-tetracycline plates in the morning.


  • In the afternoon, I plated 10 of the successful colonies that were transformed during the weekend. I left them incubating at 37°C ON. The plates are as follows:


1. Luciferase with J23106 (ampicilin).

2. Luciferase with plasmid 18 (tetracyclin).

3. Mutated luciferase (red) with J23106 (ampicilin).

4. Mutated luciferase (red) with plasmid 18 (tetracycline).