User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/03

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Repeating the transformation


  • Today, I made the transformation of the ligation realized yesterday. The resulting strains were plated in LB agar and left to grow for 8 hrs. The plates with their respective strains are:


1. Ligation of luciferase and double terminator (T)in a LB with kanamycin plate.
2. The ligation control (plasmid 18 already cut with ) in a LB with tetracycline plate.
3. The transformation control (pSB1T3 with an insert of RFP) in a LB with tetracycline plate.
4. Control of competent cells in a LB with tetracycline plate.