User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/05/19

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05/19/2010: Repeating luciferase purification... FAILED


  • Today, I started the day by repeating the DNA purification from a gel band using low melting point agarose 2%. After electrophoresis at 95V for 1 hour,I purified the band as previously done. Looking forward to observing the purified luciferase, I ran another gel; however... the band was not there!!!


  • Tomorrow, I will ask Miguel for advice to run a PCR, as the purification will probably not give any output as far as the previous results indicate.


  • I also made petri plates, 13 of LB with ampicilin and 12 of LB with kanamycin.