User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/05/06

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May 6th, 2010


  • Previous to ligation, I dephosphated the double terminator BBa_B0015 with the following reaction for 30μL:


-H2O --------------> 11μL
-Buffer -----------> 3μL
-DNA -------------> 15μL
-Phosphatase --> 1μL

  • The reaction was incubated at 37°C for 20 min and at 65°C for 10min.


  • I observed a clear band by electrophoresis in a 8% agarose gel.