User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/05/05

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Repeating the luciferase band purification

  • I repeated what I did yesterday (restriction and electrophoresis). This time, I was able to purify a 414.4μg band of ~1600bp, which is consistent with the described 1653bp of luciferase.

  • After following the protocol, I ended with a 50μl tube with purified luciferase ready for ligation.

  • I ended by doing a restriction assay with ECO R1 and XBA1 of the double terminator BBa_B0015 (the one augusto had worked in), and left it at 37°C ON.

  • Tomorrow, I will do the ligation.