User:Maria Briscione/Notebook/Chem 571/2012/02/22

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  • bsa+ gold and dye with fluorescence 1st batch (400-600 nm and 280)
  • BSA + dye (same protocol from the 2/14/12)
  • BSA + buffer for the concentration curve as a standard


BSA + Buffer

  • 406µl buffer/ 2776 µL = x / 10000µL
  • (15µM)(2.7)=(x)(3.18) new concentration in the BSA+Dye+buffer solution = 12.7 µM of BSA in buffer +dye
  • diluted gold to 30 µL gold+BSA+dye+buffer +1,000 µL buffer
  • diluted gold to 200 µL gold+BSA+dye+buffer + 1,000 µL buffer
  • BSA+buffer - diluted 30 µL BSA+buffer + 1,000 µL buffer


  • Data was lost


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