User:Maria Briscione/Notebook/Chem 571/2012/02/14

Biomaterials Design Lab

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Objective Fluorescence of varying concentrations of dye in distilled water / dye in buffer Testing fluorescence of the BSA+DYE+buffer test fluorescence of blank (buffer) Concentration Curve Description Stock Solutions: 50µM of dye in phosphate buffer 0.001 grams of dye + 50 ml buffer 50 µM of dye in distilled water 0.001 grams of dye + 50 ml buffer Varying Concentrations of Dye – Concentration curve Add 200µL of stock to 800µL of buffer = 10 µM Add 100µL of stock to 900µL of buffer = 5 µM Add 20µL stock to 980 µL of buffer = 2 µM Resulting Concentrations 1µM, 2µM, 5µM, and 10µM 13µM(30/1000) = .381 µM of dye in the dye+BSA Data Notes it appears to be a shift for the BSA + dye