User:Maria Briscione/Notebook/Chem 571/2011/11/08

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Objective

Description

Protein Expression

medium- 4 flasks containing:

  1. 10 grams tryptone
  2. 5 grams yeast extract
  3. 2 g glucose


  • 4.5 mL (100 mg/mL) Ampicillin = 0.269 M
  • 4 mL .1 M IPTG = .095329 g IPTG / 4 mL DH2O
  • add 25 µL of ampicillin to each flask

________________________________

Nanoparticle Synthesis

  • do dirty test tubes provide nucleation sites for growth?
  • want gold nanoparticles to not be tied up on a fiber (protein aggregates)- spread out through solution


  • gold solution - 100 µL
  • BSA (10 mg/ 10 mL water) - 100 µL BSA
  • 800 µL water


  • epitubes (1/10 volumes)
  • 2 small centrifuge tubes with same solution were also used and were left in heat block- stayed purple the entire time then when taken out of heat the fibers started to form in a purple solution
  • heat block 70 degrees C


  • tube 1- stays in oven
  • tube 2- in for 50 min / out for 10 min

__________________________________

Mini Preps

  • 5 mL LB
  • 5 µL amplicillin
  • colony
  • resuspend cells (5µL) and place in centrifuge tube
  • 250 µL cell lysis invert 4 times
  • incubate 5 min
  • 10 µL protesease and invert 4 times
  • incubate 5 min
  • 350 NBS
  • centrifuge for ten minutes

Data

  • Add data and results here...

Notes

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