User:Maria Briscione/Notebook/Chem 571/2011/11/02

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The purpose of the present experiment was to synthesize gold nanoparticles and to transform DNA.


Gold Synthesis

1 mL choloroauric acid (2.5 mM) + 1 mL BSA (15 µM) + 8 ml distilled water (new cholorauric acid). = diluted to .25 mM and 1.5 µM


PCR prep:

  1. DPN1 was added to to the PCR products to "chop the non PCR DNA"
  2. 10 µL of the DNA was added to a separate tube
  3. 2 µL of loading buffer was added to the tube

The solution was then loaded into the gel and was run


LB Agar Plates:

  1. .875 g LB
  2. .7 g Agar
  3. 35 mL distilled water


Transforming DNA:

  1. The cells were then plated on LB/agar plates with a 35 μL of antiobiotics
  2. 30 μL of competent cells
  3. 5 μL of DNA were combined and incubated on ice for 30 minutes
  4. The DNA/cell mixture was then heat shocked at 42C for 30 sec
  5. incubated on ice for 5 min
  6. 250μL of SOC media was added to the mixture
  7. incubated in the shaker at 37C for 1 hr
  8. 100μL of cells were spread (using sterile techniques) on the LB/agar plate
  9. The plate was then inverted and stored in a 37C oven overnight


"fresh" chloroauric acid was seen and a purple color was seen after the solution was taken out of the oven the second to last time.


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