User:Maria Briscione/Notebook/Chem 571/2011/08/31
|Biomaterials Design Lab||Main project page|
The purpose of the present subset of experiments was to monitor the change in concentration of AuNPs.
Bovine serum albumin (BSA, 0.0015-0.015 mM), a weak reducing agent, was mixed at room temperature with HAuCl4 (0.25-1mM) in aqueous phase. For 6 hours the mixture was maintained in a thermostat at 40, 60, or 80 °C. A color change of colorless to pink then purple was observed and indicative of the reduction. After 6 hours, the samples were first cooled, kept overnight, and purified twice to remove excess BSA. Specifically, AuNPs were collected at 10000-14000 rpms for 5 minutes after washing with distilled water.
UV spectrophotometer recorded UV- visible spectra of the prepared gold colloidal suspensions and a time dependent factor was determined by performing time dependent scans of choice reactions. Additionally, temperature monitoring over 20-70 °C range determined whether temperature was a significant factor. The pH of the samples was also determined. The various reaction solutions were contained in glass flasks that contained a double walled jacket. The temperature was maintained by use of externally circulating water while pH measurements were taken from a pH meter at regular increments for 6 hours.
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