User:Marcel Quint/Notebook/wai cloning/2008/04/25

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Owwnotebook icon.png wai cloning Report.pngMain project page

investigate methods to pcr/sequence from T-DNA into genomic DNA

  • plasmid rescue
  • walking in uncloned genomic DNA

→ settled on method by Siebert et al. (1995) NAR using adaptors that prevents amplification of double-stranded adaptor sequences at both ends; this method has been progessively developed into the genome walker kit by clontech → try self-made without kit