User:Marcel Quint/Notebook/medicago/2008/04/24

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check competent cells

  • transform empty pRedroot vector into competent cells by electroporation, dissolve in 3 ml LB and plate the following dilutions on LB kan/spec plates: 2-5-50μl + spin down, dissolve in 150μl and plate everything → check on sat

use this protocol - link still missing