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Hot SHOT testing

  • material: 13 p00 mouse limbs of RL119 and RL120
  • incubation: 75µL/limb, 30 min
  • concentrations: range from 0.4 to 4.24 µg/mL (avg = 1.67; median = 1.75) [1]
  • PCR: Green GoTaq (regular AWS program)
  • bands showed up pretty well, although weaker then with protK, and required photoshopping before reading
rl119: a-e reelers; f het
rl120: a,e,f reelers; b,g het, c,d inconsistent with ProtK method

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