User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28
|Experimental Biological Chemistry: Fall 2013||Main project page|
August 28, 2013
Execute procedures from from Dr Harting's AU Biomaterials Design Lab Notebook
[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> Final [HAuCl4]: 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> <br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> <br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> <br.> Rest of procedure followed as written.
[HAuCl4]=0.249Mm<BR.> Sodium citrate=1.01%<br.> <br.> Rest of procedure followed as written.<br.> <br.> Final volume AuNPs: 25.6mL<br.> <br.> Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.>