User:Mackenzie K. Kelley/Notebook/Experimental Biochemistry/2017/10/24

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Objectives Wash, filter, characterize clays using XRD and IR Made more AuNPs supported by TiO2 Used Au/TiO2 to selectively oxidize glycerol Prepare clay supported AuNP using each clay Description Wash, filter, characterize clays Used 1.0 um nylon filter paper to filter clay Rinsed clay with DI water and ethanol 5 times Spread clay on watchglass in a thin layer and dry in oven at 105 C overnight Ground clay to a fine powder in a pestle & mortar to use for characterization ran XRD and measure x-ray between 2 degrees and 50 degrees Measured IR of clay samples Prepare clay supported AuNP using each clay place 0.3 g of clay in a small glass vial add 3.4 x 10-5 mol HAuCl4 (3.4 mL of 10 mM HAuCl4) add 16.6 mL of water stir until solution changes color (overnight may be necessary) repeat for each clay sample Use Au/TiO2 to selectively oxidize an glycerol place 0.2 g AuNP in 100 rbf add 1 g glycerol and 29 mL methanol add 0.75 g of NaOH place balloon on 100 mL rbf to introduce O2 into the reaction react for 2 hr remove O2 balloon & centrifuge to separate & recover Au/TiO2 save fluid for analysis next week if you have time, filter & wash Au/TiO2 using nylon 0.2 um filter Made new clay supported AuNP from last days procedure