User:Mackenzie K. Kelley/Notebook/Experimental Biochemistry/2017/10/03

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Objective

  • Run ICP on 5 ICP standards for Au and gold stock solutions to measure the [Au] in our stock solutions
  • Remake D2O solutions using dried AuCl3 solutions for each amino acid

Description

  • Prepared 5 ICP standards for Au (in range of 0 - 200 ppm)
  • Diluted all HAuCl4 stock solutions to range of 100 - 1000 uM
  • Ran ICP on 2ml samples of stock solutions, AuNP colloids, & AuNP fiber solutions
  • Remade D2O solutions (one for each AA) for tomorrow using dried AuCl3 solutions
  • Used vial of dried Au and added 1 mg AA, 0.1 mL NaOH in D2O, and 0.9 mL D2O
  • Measured UV/VIS of AuNP solutions that remained stable

Results

Screen Shot 2017-10-24 at 11.54.57 AM.png

We used the Au-R 242.794nm column to calculate stock solution concentrations.

calculation used to find actual molarity of gold in gold stock solution:

86.15ug/mL*1000mL/L*1g/10^6ug*1mol/196 = 4.395*10^-4 M

Our "2.5mM" stock solution was actually 2.19 mM.

Our "10mM" stock solution was actually 9.075 mM. The gold nano-fiber vs nanoparticle concentrations of gold in solution were as expected with the nanoparticles having a much higher concentration than the nano fibers.