Objective
- Dialysis on a AuNP solution to determine:
- how big the AuNP complexes are
- how strongly the stabilizer is bound to the AuNP
- if we can change the stabilizer
- how quickly the molecules migrate through the tubing
- Remake AA solutions (didn't work last time)
Description
- prepared and ran dialysis on two dialysis tubes: 1 with DI water and 25 mM HdMe2ImCl, 1 with AuNP solution and 25 mM HdMe2ImCl (Cysteine in BSA 90 ratio Au:AA)
- Ever 30 minutes, remove 1 mL of solution from jar and measure the absorbance at 211 nm using the spectrophotometer
note: we were taking the solution out of the dialysis bag itself
- Using same procedure to prepare dialysis tubes, prepared a total of 4 dialysis tubes.
- Dialysis 3 mL AuNP stabilized with basic AA against Glycine buffer
- Dialysis 3 mL AuNP stabilized with basic AA against water
- Dialysis 3 mL AuNP stabilized with BSA against Glycine buffer
- Dialysis 3 mL AuNP stabilized with BSA against water
Data
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