User:M Jaffe/Notebook/Maxis Protease Lab 471/2015/12/01

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Objective

As a class we developed standard procedure for running ICP, conductivity and UV-Vis experiments for the following day and prepared the respective samples.

Procedure

1. Dr. Hartings prepared 20 mL AU fibers for the class. Procedure Here

2. We made the following samples

Sample Concentration (of Trypsin) Amount Buffer Amount of Trypsin
1 1.0 uM 19.7425 mL 0.2575 mL of 77.68 uM
2 1.0 uM 19.7425 mL 0.2575 mL of 77.68 uM
3 1.0 uM 19.7425 mL 0.2575 mL of 77.68 uM
4 0.1 uM 19.9742 mL 0.02575 mL of 77.68 uM
5 0.1 uM 19.9742 mL 0.02575 mL of 77.68 uM
6 0.1 uM 19.9742 mL 0.02575 mL of 77.68 uM
7 0.01 uM 19.99 mL 0.01 mL of 1.0 uM
8 0.01 uM 19.99 mL 0.01 mL of 1.0 uM
9 0.01 uM 19.99 mL 0.01 mL of 1.0 uM
10 0.001 uM 19.999 mL 0.001 mL of 1.0 uM
11 0.001 uM 19.999 mL 0.001 mL of 1.0 uM
12 0.001 uM 19.999 mL 0.001 mL of 1.0 uM
Supernatant
1 no Trypsin 0 0
2 no Trypsin 0 0
3 no Trypsin 0 0

3. We incubated all samples in a 37 degree C water bath for approximately 21 hours.