User:M Jaffe/Notebook/Maxis Protease Lab 471/2015/09/29
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ObjectivesConduct Bradford analysis on protease (trypsin) degraded samples. Instructions can be found here.
ProcedureIn order to do the Bradford Assay of Protease Degradation, we followed Dr. Hartings' protocol, and did the following specific steps:
Calculations: V1 = [(1µM)(1mL)]/46.35µM = 0.02157mL, amount of trypsin solution needed Volume of buffer: 1mL - 0.02157mL = 0.9784mL Data |