User:Linh N Le/Notebook/2009/08/11

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To Do Incubator Motillity? Incubator Koch wants me to put a jug of water in the incubator for a couple of hours and see what the temp is Since it is already set to max (60) i will leave it there and see if the water gets too hot Its been ~1hr and the incubator is set to 60. The water in the beaker (which i placed on the far side away from the fan) is reading 40-41C. Thats too hot, so i reduced the incubator to 55 and changed out the water. The incubator set at 55 yielded water at 39 Trying setting of 50 50 yields 35C water Trying 52.5 A note is that i have the beaker furthest from the fan. The booklet says that there is a temperature gradient, so close to the fan=hotter At 52.5, on the far side of the chamber, the water reads 37c! Motility Andy mentioned yesterday that we may do some microscope stuff He may want to try his lipids on some microtubes He spin coated a small cover glass with the lipds Half of the slide was covered with tape We put MT's on the slide and looked at it under the scope We saw MT's stuck to the glass but not to the lipids (as wanted) We used the old kinesin Andy drummed up from yesterday, but with no luck