User:Linh N Le/Notebook/2009/07/13

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To Do Autoclave stuff today! Pipette tips (1000ul) that fell out, they are sitting in the autoclave More centrifuge tubes (the large ones) Popin buffer More BNC Cables OT? Autoclaving When I came in, the Autoclave was in use Andy says that Nathan (Osinski's Lab) was using it Round one (probably the only round since our group meeting is today) 1x 1000ul tips (the ones i got dirty Friday) 2x 100ul tips 5x large centrifuge tips Note: I went ahead and autoclaved ALL of the tubes in the plastic bag since we only had 1 more container of them and I wanted to make sure we had some in reserve Round 2 The "popin buffer" BNC Whilst waiting for the Autoclave, I will make some 3ft and 4 ft BNC cables one 4ft made friday, one made today two 3ft made today New Lab Network Used Koch's laptop to check the 4 ports that are in the new lab (2 at front door 2 at rear door) They all provided me with internet access and I was able to access //controller I will take down the annoying wire tomorrow since today I need to read up on the paper for the meeting OT Koch, Ant and I tethered some DNA and tried unzipping There is a problem with the translator/piezzo They seem to be misaligned because we get alot of "sliding" Ex. the x translation works fine, but when you use the Y translator it has a diagonal movement wrt the microscope plane We were able to find some tethers and pull on them We feel that the trap is too weak since the beads keep coming out of the trap it could also be a problem with the piezzo (misaligned and/or moving too fast) Good note: 500nm beads are easy to see and very easily trapped (sometimes we get them when we dont want them) We need to calibrate the tweezers tomorrow Cool note: I think i saw dna unzip, but that result was unrepeatable We turned on the trap near a tether, saw it move, then turned off the trap. The bead "sprung back" to its original spot and oscillated a bit (like a block on a spring)