User:Klare Lazor/Notebook/Chem-496-001/2011/11/08

Biomaterials Design Lab

Main project page Previous entry      Next entry

Objective Description Protein Expression medium- 4 flasks containing: 10 grams tryptone 5 grams yeast extract 2 g glucose 4.5 mL (100 mg/mL) Ampicillin = 0.269 M 4 mL .1 M IPTG = .095329 g IPTG / 4 mL DH2O add 25 µL of ampicillin to each flask ________________________________ Nanoparticle Synthesis do dirty test tubes provide nucleation sites for growth? want gold nanoparticles to not be tied up on a fiber (protein aggregates)- spread out through solution gold solution - 100 µL BSA (10 mg/ 10 mL water) - 100 µL BSA 800 µL water epitubes (1/10 volumes) 2 small centrifuge tubes with same solution were also used and were left in heat block- stayed purple the entire time then when taken out of heat the fibers started to form in a purple solution heat block 70 degrees C tube 1- stays in oven tube 2- in for 50 min / out for 10 min __________________________________ Mini Preps 5 mL LB 5 µL amplicillin colony resuspend cells (5µL) and place in centrifuge tube 250 µL cell lysis invert 4 times incubate 5 min 10 µL protesease and invert 4 times incubate 5 min 350 NBS centrifuge for ten minutes Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.