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making a labeled cysteine fluorophore
Use of a distant reporter group as evidence for a conformational change in a sensory receptor
Importance of this reaction
at pH 7 makes it easy to prepare green-fluorescent thiol conjugates of biomolecules. 5- Iodoacetamidofluorescein has been one of the most extensively used visible wavelength– excitable, thiol-reactive dyes for modification of proteins, nucleic acids and other biopolymers in the literature. Following conjugation to thiols, fluorescein-5- iodoacetamide can be radioiodinated. Conjugates of M0638 have a high intrinsic detectability, particularly when used with instrumentation that incorporate the 488 nm spectral line of the argon-ion laser. Furthermore, its negative charge also makes capillary electrophoretic separation of labeled adducts possible. (MGT inc. Product information sheet www.markergen.com)
and ﬂuorescence maxima in the visible region of the electromagnetic spectra (λabs 490 nm and λem 512 nm, in water), is one of the most common labels used in biological applications.
Figure: Cysteine MW: 121.16g/mol
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