User:Klare Lazor/Notebook/Chem-496-001/2011/09/28

Biomaterials Design Lab

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Objective Synthesis of gold nanoparticles at a constant temperature of 80 degrees Celcius in water Description Starting concentrations for Chloroauric acid and BSA solutions: Chloroauric acid --> 17.0 mg in 10 mL of water --> 4.3 mM BSA --> 10.3 mg in 10 mL of water --> 15.4 uM Used these stocks to make solutions for synthesizing nanoparticles. Use the same final concentrations that you used on the 7th, which was .25mM Au+3 and 1.5 uM of BSA. Also, use the same buffers that your groups were assigned to. Tomorrow we will all use water for the synthesis. I will give you directions for how we are conducting this experiment at the beginning of class. 1mL of sample in cuvet cover with parafilm temperature control of uvis 80 degrees celcius Data Calculations Final concentrations from September 7th: 1mL of 0.25mM Au3+, 1mL of 1.5uM BSA, and 8mL of H20 or Buffer M1V1=M2V2 4.3E-3*V1=2.5E-5*.01L--> 581.39 uL of AU3+ 15.4E-6*V1=1.5E-6*.01L--> 974.02 uL of BSA Volume of H20 or Buffer--> 8445 uL of H20 total volume of solution: 10,000uL Charts Chart 1: Absorbance vs. time at 550 nm Wavelength Chart 2: Absorbance vs. wavelength for all times Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.