User:Khyra A. Neal/Notebook/Chem 571/2014/09/24
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September 25,2014High Pressure Liquid Chromatography (HPLC)Running HPLC with Gel Permeation Column (GPC) Tasklist1. Prepare 0.5 g/L and 0.1 g/L Lysozyme from 1 g/L Lysozyme Stock
2. Prepare 0.5 M acetic acid and 0.3 M Na2SO4 HPLC Solvent
3. Filtered 0.5 M acetic acid and 0.3 M Na2SO4 by membrane filtration to removed particles and remove CO2 4. We ran our yellow standard and Lysozyme samples at 0.8 mL/min for 12 minutes initially. We noticed that all of the sample didn't leave the column with the time frame so we extended the remaining samples up to 16 minutes. We also ran our [30]:[1] Lysozyme colloid sample at 0.8 mL/min for 16 minutes. All of the samples were injected into the HPLC using a syringe and syringe filter.
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