User:Khyra A. Neal/Notebook/Chem 571/2014/09/03
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September 3, 2014
Titration of Amino Acids
Today we continued with our amino acid titrations. We started with the second trial of Isoleucine and Histidine,two trials of Tyrosine, and one trial of Alanine.
The figures below show the titration curves of amino acids.
NOTE Tyrosine was dissolved in a 1:10 1.0 M HCL and H2O solution because tyrosine is aromatic and hydrophobic so a stronger acid was need to dissolve it before titration. The titration curves for Tyrosine are inaccurate because as the titrant was added to the solution a white precipitate formed. It may have formed because the concentration of Tyrosine had reached its maximum solubility for the solution at hand. The second trial for tyrosine was stopped prior to the pH reaching 10 because a large amount of precipitate was formed.
200 μM Amino Acid Solutions