User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/24

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Purpose

  • DAY 8 - Continue with running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.

Procedure

  • The flasks were taken out from orbital shaker.
  • 3mL of cell culture were taken from each flasks in a 4mL glass cuvette.
  • The absorbance for the 3mL cell culture was taken with wavelength 600.0nm.
  • Absorbances were recorded.

Results

  • A graph of absorbance versus number of days for which the films were soaked in media was plotted.
  • The absorbance measured on day 2, 4, 6 and 8 indicates the antibacterial activity exhibited by the media after silver-incorporated films has soaked for 2, 4, 6, or 8 days. All measurements were taken once, thus no error bars are displaced on graph.

8-day study on cell growth.png

Observation

  • From the graph, the flasks containing control film and the flasks containing only cells and media all exhibited high absorbance through the 8-day experiment, while the flask containing silver-incorporated film exhibited antibacterial activities for day 2, 4, and 8.
  • The absorbance for both control flask and flask containing control films reached between 2.0 to 2.5 after 15-hours of cell growth. This indicates that there were no antibacterial effects within both control flasks. However, the absorbance for flasks containing 33% silver-incorporated film was low for day 2, 4, and 8. The absorbance after 15-hours of cell growth peaked on the 6th day. This showed that there was antibacterial growth in film containing silver. The maximum amount of antibacterial growth is on day 2.