User:Katie C. McCallum/Notebook/BLI-3/p38 MAPK pathway/2011/06/24
|Project name||Main project page|
Froze down OP50, trf-1 (1), and trf-1(2) stock. (750 ul culture, 250 ul 80% glycerol)
Plasmid prep'd trf-1 (1) and trf-1 (2) and nano-dropped to get the concentration of each; 227 and 206 ng/ul, respectively.
Plated 300 ul of OP50 on 12 plates and incubated in 37C
Froze down gst-4::gfp, KJ142, and VZ1 (1 ml wash product + 600 ul S buffer + 30% glycerol)
Cracked gst-4::gfp plates (3) and incubated at RT on rotisserie shaker.