User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14

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Benchwork

Plan

(See Results below)

  1. Transform again
  2. re-plate remaining 800μL of transformation from yesterday
  3. Start miniprep culture of single colony

Experimental

  1. Transformation of pTXB1+His QuikChange into DH10B
    1. 5μL QuikChange experimental reaction from Friday (Daniel Catt's + 200μL DH10B
    2. 5μL QuikChange control reaction from Friday (Daniel Catt's + 200μL DH10B
  2. Re-plate transformation from yesterday
    1. spread 800μL pTXB1+His/DH10B QuikChange experimental transformation from yesterday on LBAmp plate
    2. spread 800μL pTXB1+His/DH10B QuikChange control transformation from yesterday on LBAmp plate
      • → 37°C O/N
  3. Miniprep
    1. 10 mL Muratore:Materials/Media/LB + 10 μL 100mg/mL Amp + pTXB1+His/DH10B QuikChange experimental transformation colony from plate (colony #1)
      • → 37°C O/N with shaking

Results

  • Single colony on experimental plate from yesterday
    • → pTXB1His/DH10B QC colony #1
    • No colonies on control


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