Benchwork
Plan
(See Results below)
- Transform again
- re-plate remaining 800μL of transformation from yesterday
- Start miniprep culture of single colony
Experimental
- Transformation of pTXB1+His QuikChange into DH10B
- 5μL QuikChange experimental reaction from Friday (Daniel Catt's + 200μL DH10B
- 5μL QuikChange control reaction from Friday (Daniel Catt's + 200μL DH10B
- Re-plate transformation from yesterday
- spread 800μL pTXB1+His/DH10B QuikChange experimental transformation from yesterday on LBAmp plate
- spread 800μL pTXB1+His/DH10B QuikChange control transformation from yesterday on LBAmp plate
- Miniprep
- 10 mL Muratore:Materials/Media/LB + 10 μL 100mg/mL Amp + pTXB1+His/DH10B QuikChange experimental transformation colony from plate (colony #1)
Results
- Single colony on experimental plate from yesterday
- → pTXB1His/DH10B QC colony #1
- No colonies on control
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