User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14

AU CHEM-570 Lab Prep

Main project page Previous entry      Next entry

Benchwork Plan (See Results below) Transform again re-plate remaining 800μL of transformation from yesterday Start miniprep culture of single colony Experimental Transformation of pTXB1+His QuikChange into DH10B 5μL QuikChange experimental reaction from Friday (Daniel Catt's + 200μL DH10B 5μL QuikChange control reaction from Friday (Daniel Catt's + 200μL DH10B Follow standard transformation protocol, except increase heat-shock time to 45s Plate 200μL on LBAmp and grow O/N at 37°C → store remainder @ 4°C Re-plate transformation from yesterday spread 800μL pTXB1+His/DH10B QuikChange experimental transformation from yesterday on LBAmp plate spread 800μL pTXB1+His/DH10B QuikChange control transformation from yesterday on LBAmp plate → 37°C O/N Miniprep 10 mL Muratore:Materials/Media/LB + 10 μL 100mg/mL Amp + pTXB1+His/DH10B QuikChange experimental transformation colony from plate (colony #1) → 37°C O/N with shaking Results Single colony on experimental plate from yesterday → pTXB1His/DH10B QC colony #1 No colonies on control