User:Karmella Haynes/Notebook/miR Trigger/2011/07/24

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07/24/11

  • Transfection: plate set-up for miR-34 and miR-luc sensors for real time microscopy (tomorrow at 12 pm)



Transfection/ real time microscopy plate
> Use 12-well glass bottom plate and phenol red-minus media (FBS-ts and P/S, no ab)
> Wells 1-4, U2OS plain
> Wells 5-12, JDS33-47
> ~200k cells per well; 1 mL medium each