User:Karmella Haynes/Notebook/miR Trigger/2011/06/11

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06/11/11

  • ✓ YFP/miR-luc time course: Plate cells and start dox induction



YFP/miR-luc time course
> Plate JDS33-47 cells at ~1x105 in 3x 12-well plates; use U2OS plain medium
> Set up three wells per time point for triplicate trials
> +Dox (1μg/mL) time points:

  1. Sat, 6/11/11, 12 pm (55 hrs.)
  2. Sat, 6/11/11, 7 pm (48 hrs.)
  3. Sun, 6/12/11, 7 am (36 hrs.)
  4. Sun, 6/12/11, 7 pm (24 hrs.)
  5. Mon, 6/13/11, 7 am (12 hrs.) (9:30 am)
  6. Mon, 6/13/11, 10 am (9 hrs.)
  7. Mon, 6/13/11, 1 pm (6 hrs.)
  8. Mon, 6/13/11, 4 pm (3 hrs.)
  9. no dox

--> Harvest cells at 7 pm for Flow Cytometry