User:Karmella Haynes/Notebook/miR Trigger/2011/01/24

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01/24/11

  • ✓ Transfections: check RFP expression of Repressor-transfectants
  • ✓ Cultures: thaw JDS33-47; mine 4/2010, and one from Joe 2/2010; use 20%FBS medium



RFP expression
> Saw same amount of RFP expression from all 6 plasmids
> RFP from reverse constructs shows that polyA BioBrick works (or at least does not stop expression)
> Continue with miR circuit consruction by placing CFP-Target genes in front of Repressors