User:Karmella Haynes/Notebook/miR Trigger/2011/01/17

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01/17/11

  • ✓ Planning: miR trigger construct tests



miR trigger constructs
> Fun with control constructs and a truth table

File:KAH 011711 circuit.tif
--> Note: R and T are driven by constitutive promoters
--> R = RFP fluorescence; T = CFP fluorescence


miRNA m r Output
- - - R T
+ - - R T
- + - R T
- - + R
+ + - T
- + + R (off state)
+ - + R
+ + + T (on state)


--> +/- m or r constructs will be built by including or excluding the miRNA target or Gal4 binding DNA elements
--> Should be able to control luc miRNA with dox (JDS33-47 cells); if too leaky, use U2OS plain cells