- ✓ New idea: three-color miR sensor to report different levels of miR expression
- ✓ Order plasmid: phGluc (22522) from Addgene (to make new miR-luc gene, w/o YFP)
New idea: three-color miR sensor to report different levels of miR expression
> Scheme: RFP-Repressor-miRtarget silences YFP-10xGal4-Reporter #1 and CFP-15xGal4-Reporter #2
--> Will need to experiment with varying Gal4 copy number to get different sensitivities
> Need to modify miR-luc gene so that it does not need YFP
> Use secreted reporter Gaussia luciferase, mammalian expression optimized (see http://www.addgene.org/pgvec1?f=c&identifier=22522&atqx=Gluc&cmd=findpl)
--> Has no BB sites!