User:Karmella Haynes/Notebook/miR Trigger/2010/05/03

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05/03/10

  • CFP Reporter + JDS33-47 lines: puro selection plates



CFP Reporter lines
> Set up puro selection plates (25 mL) for two lines (FC# and FC#)

  1. 1.0 μg/mL puro
  2. 1.5 μg/mL puro (JDS33-47 parent line is puro resistant, try increased puro)

> ~100,000 cells per plate
> 6-well back-up non-clonal plate (1.0 μg/mL puro)