User:Karmella Haynes/Notebook/Polycomb project/2011/07/17

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07/17/11

  • ✓ Transfection: Plate HEK Flp-in T-REx to test inducible TK-Cyan reporter constructs; 1:5 dilution from 100% confluent 10 mL flask; 1 mL/well, 12-well plate
  • ✓ Kill curve: test hygromycin on HEK Flp-in T-REx



Kill curve: hygro, HEK Flp-in T-REx
--> 12-well plate, 1 ml/ well
--> Results 7/21/11

  1. 0μg/mL:
  2. 5: normal growth
  3. 10: normal growth
  4. 20: normal growth
  5. 50: mostly normal growth
  6. 100: A lot of death, but some cells still sticking
  7. 200: death
  8. 400: death
  9. 500: death
  10. 600: death
  11. 700: death
  12. 800: death