User:Karmella Haynes/Notebook/Polycomb project/2011/04/11

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04/11/11

  • ✓ Luc assay: transfections from 4/09/11
  • ✓ RNA prep: remainder of transfected cells (Note: yield too low to make good cDNA; do RNA isolation on whole sample next time)



Luc assay

  • Resuspend cells in 1 mL growth medium (do not aspirate or add new med.)
  • Collect 500 μL from each sample
  • Use 3x 100 μL for Bright-Glo luc assays
  • Use 25 μL cells (diluted in 75 μL DPBS) for cell counts (200 ng transfection samples only)