User:Karmella Haynes/Notebook/Polycomb project/2011/03/11

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03/11/11

  • ✓ Luc activity assay: 12-day luc rep, 2-day Pc-TF



Luc Activity Assay

Luc activity assay > Cells: dox-induced Gal4-EED expression/ 2 days, followed by transfection for 4 days
1-6. KAH160/MV1 (human Pc-TF), 0, 50, 100, 200, 400, 800 ng plasmid
7-12. KAH170/MV1 (human Pc-TF), 0, 50, 100, 200, 400, 800 ng plasmid
> Sample processing:

  • Add 500 μL medium to cells in rep 1 set (measured before); Resuspend cells in 1 mL medium total
  • Aliquot 3x 100 μL samples into 96-well plate
  • Save the rest for cell count (Millipore Scepter)
  • Normalize each value to cell count