User:Karmella Haynes/Notebook/Polycomb project/2011/01/29

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01/29/11

  • ✓ U2OS plain: split 1:10
  • ✓ JDS33-47: refresh medium (U2OS plain, 1x FBS); cells starting to recover from thaw, after some coaxing with 2x FBS
  • ✓ HepG2: expand to 75 cm2 flask
  • ✓ HEK293 #4 Luc: very weak growth after thaw; request back-up from Mirra
  • ✓ Gal4-EED 23;4;9: great growth; will give "10/3/10" frozen aliquot to Manching