User:Karmella Haynes/Notebook/Polycomb project/2010/11/21

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  • ✓ HEK Gal4EED: split stocks 1:5 (puro maint. medium)
  • ✓ HEK Gal4EED induction: split 10 cm plates 1:5; 11/19 dox plate, refresh dox; no dox plate - make two new plates, add dox to one
  • ✓ HepG2 clones: split clones into two 24-well plates; be sure to resuspend very well w/ micro-pipetter, cells tend to clump