User:Karmella Haynes/Notebook/Polycomb project/2010/11/17

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11/17/10

  • ✓ HepG2 TREx line: pick clones, 24 total; grow in 24-well plate
  • ✓ HEK293 Gal4-EED: split 1:5 (two 75 cm2 flasks); split 1:10 in 10 cm plates for dox induction tomorrow
  • ✓ Plasmid transformation: Quick & dirty re-transformation for Gal4-ATF test constructs KAH59/VNpc, 54/VNpc, 66/Npc, 65/Npc, 59/Npc (no need to do 60/VNpc and 36/VNpc, already have enough for transfection); transform pcDNA6 (TREx)