User:Karmella Haynes/Notebook/Polycomb project/2010/08/23

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  • ✓ Cell culture: split confluent lines
  • ✓ Transfection: H3me3 reporter lines, set up 12-well plates for Fugene transfection of Pc-ATF's
  • ✓ Microscopy: set up 24-well glass cover slip plates for KAH130-4, 150-8, 131-9, 159-2 (6 wells each)
  • ✓ ChIP/ Co-IP: expand KAH130-4 and 131-4 to 25 mL plates (plain medium), 5 plates each