User:Karmella Haynes/Notebook/Polycomb project/2010/08/09

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08/09/10

  • ✓ Senescence assay: set up bio replicate #3 for -/+ dox KAH126-1, 126-3, 132-8, 154-2, FTRx (DMSO, rotenone, dox, u.t.)
  • ✓ Cultures: split confluent plates 1:10
  • ✓ Microscopy: treat KAH130, 131 samples with 1 ug/mL dox
  • ✓ Western: treat KAH130, 131 samples with 1 ug/mL dox
  • ✓ H3me Reporter line screening: split new 12-well plates (previous ones too crowded for transfection)
  • ✓ Midiprep cultures: for Casey's ES cell transfections (see Biobrick cloning notebook)